Part:BBa_J72033:Design

{Bmll70} in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

N/A

Source

Overlap Extension PCR (Wobble Construction)

Wobble construction of Bmll70 {<Strep!}

Wobble mll70/mll71                (61, EcoRI/BamHI
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll70         {<Strep!}
----
mll70    Forward wobble for {<Strep!} (Bmll70)
CCATAGAATTCATGAGATCTacctggagccacccgcagttcgaa
mll71    Reverse wobble for {<Strep!} (Bmll70)
CGTTAGGATCCttatttttcgaactgcgggtgg